luciferase相关论文
Tumorigenesis and spontaneous metastasis by luciferase-labeled human xenograft osteosarcoma cells in
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Scribble(Scrib)是富含亮氨酸重复序列及PDZ结构域蛋白(LAP)家族的一员,以脚手架蛋白的方式与多种分子相结合并整合多种信号通路协......
Objective:This study observed attenuating effect of hydroxysafflor yellow A(HSYA),an effective ingredient of aqueous ext......
AIM:To confirm the role of sex-determining region Y-box 7(Sox7) in aspirin-mediated growth inhibition of COX-independent......
Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its b......
目的构建能表达荧光素酶的重组柯萨奇病毒B组3型(coxsackievirus B3, CVB3),以实现定量检测CVB3。方法将荧光素酶基因克隆至CVB3基......
EDAG基因是本实验室分离克隆的一种与造血细胞增殖分化密切相关的新基因。EDAG基因是从4月龄人胎肝组织与成年肝组织的差异表达基......
学位
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
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目的 构建携带萤火虫荧光素酶(Luc)报告基因的腺病毒载体(Ad-Luc),研究其感染大鼠骨髓间充质干细胞(BMSC)后的体内外生物发光成像.......
A mouse model based on replication-competent Tiantan vaccinia expressing luciferase/HIV-1 Gag fusion
Background Developing an effective vaccine against human immunodeficiency virus type 1 (HIV-1) remains a grand challenge......
Tumorigenesis and spontaneous metastasis by luciferase-labeled human xenograft osteosarcoma cells in
Background Osteosarcoma(OS)is the most common primary malignant tumor of bone.Mouse models of human OS can invariably pr......
为实现基因治疗过程中的有效药物筛选及体内检测,首次利用核糖体内部进入位点(IRES)构建了同时携带06-烷基鸟嘌呤-DNA 烷基转移酶(......
期刊
目的 构建及验证荧光素酶标记ROP18过表达弓形虫ROP18?Ty1?LUC RH株,为研究弓形虫毒力及相关宿主免疫调控机制奠定良好基础.方法 ......
Improvement of Chemically-activated Luciferase Gene Expression Bioassay for Detection of Dioxin-like
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Improvement of Chemically-activated Luciferase Gene Expression Bioassay for Detection of Dioxin-like
To improve the chemically-activated luciferase expression (CALUX)bioassay for detection of dioxin-like chemicals (DLCs) ......
目的 为研究p53家族调控细胞凋亡的机制,构建并鉴定插入人Puma基因的启动子片段的荧光素酶报告基因载体.方法 采用PCR技术从人类He......
Current serum neutralization assays based on the inhibition of the eytopathic effect(Nt-CPE) need to manipulate live vir......
在体光学分子影像作为一种快速发展的生物医学影像技术,以其实时、定量及非侵入性等特点,已被广泛应用于各学科的研究中。光学分子影......
为实现基因治疗过程中的有效药物筛选及体内检测,首次利用核糖体内部进入位点(IRES)构建了同时携带06-烷基鸟嘌呤-DNA 烷基转移酶(......
期刊
目的:为研究10 kb以上DNA疫苗质粒的体内电穿孔递送,构建萤光素酶报告基因表达质粒pEE14.1-luc,并验证其表达。方法:从质粒pGL3-CMV中......
在研究胰岛素(Ins)、地塞米松(Dex)和甲基异丁基黄嘌呤(Mix)对脂肪细胞分化过程中PAI-1基因表达的影响基础上,为进一步探讨Ins、De......
萤光素酶是一类在氧气存在下能催化其底物特异性发光的酶,检出灵敏度高、特异性强.萤光素酶催化底物发光因无须外源光的激发,避免......
A mouse model based on replication-competent Tiantan vaccinia expressing luciferase/HIV-1 Gag fusion
Background Developing an effective vaccine against human immunodeficiency virus type 1 (HIV-1) remains a grand challenge......
目的构建大鼠酸敏感离子通道1(ASIC1)基因启动子荧光素酶报告质粒pGL3-ASIC1-promoter,并进行功能的鉴定。方法设计、合成ASIC1启......
目的 建立一种可连续定量监测的原位前列腺癌动物模型.方法 利用分子克隆技术构建表达荧光素酶的慢病毒载体,用慢病毒将GFP-Lucife......

